DNA

Part:BBa_K2005021:Design

Designed by: Jarrod Shilts   Group: iGEM16_Vanderbilt   (2016-10-12)


Poly(2)Guanine-45


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 96
    Illegal BamHI site found at 181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To generate this artificial DNA sequence, we wrote an algorithm that generated a sequence with the desired number of motifs organized into evenly-spread regions. This initial sequence was then altered according to rules that preserved the motif structure until it met a set of constraints to make it compatible with current DNA synthesis restrictions . In addition, the sequence was designed to include sites for annealing by ActB qPCR primers to facilitate sequence-specific amplification and further analyses.


Source

Synthetic DNA

References